Interactomes: Experimental and In Silico Approaches

de Barros Dantas L.L., Brand?o M.M.

Advances in Experimental Medicine and Biology, vol 1346. Springer, Cham.


Any part of the Central Dogma of Molecular Biology (DNA replication, transcription, and translation) is based on an intricate protein–protein interaction. On this chapter, we will navigate over the techniques that enable us to construct or fulfill the gaps on an interactome study, directly using assessment of the biochemical and/or molecular machinery that allow two proteins to interact with each other; or rely on computational biology techniques to gather information on PPI from public available databases and evaluate this interaction.