Leandro Pio de Sousa^1,2*, Ludmila dos Passos e Silva¹, Matheus Pena Passos³, Juliana Lischka Sampaio Mayer³, Marcelo Mendes Brandão², Oliveiro Guerreiro-Filho⁴, Jorge Maurício Costa Mondego^5*
1 - Laboratory of Multitrophic Interactions and Biodiversity, Department of Animal Biology, Institute of Biology, State University of Campinas, Campinas, SP, Brazil.
2 - Laboratory of Integrative and Systemic Biology (LaBIS), Center for Molecular Biology and Genetic Engineering, State University of Campinas, Campinas, SP, Brazil.
3 - Laboratory of Plant Anatomy, Department of Plant Biology, Institute of Biology, State University of Campinas, Campinas, SP, Brazil.
4 - Coffee Center Alcides Carvalho, Agronomic Institute of Campinas, Campinas, SP, Brazil.
5 - Laboratory of Biotechnology and Plant-Microorganism Interaction (LABIPLAM), Center for Plant Genetic Resources, Agronomic Institute of Campinas, Campinas, SP, Brazil.

Increasing attention has been given to the host phylogeny and domestication roles in shaping plant-associated microbiomes. However, the interspecific effects of hybridization on microbial communities remain poorly understood. We investigated the effects of interspecific hybridization on the composition, diversity, ecological organization, and co-occurrence patterns of leaf-associated fungal communities in five Coffea species and hybrids between C. arabica and the other four species in the same habitat. Beta-diversity analyses showed a differentiation among host genotypes. Assignment of fungal genera to guilds indicated that fungal communities were dominated by pathogen–saprotrophs. Interestingly, Coffea stenophylla, a genetically distinct species within the same broader evolutionary clade, exhibited a higher relative abundance of pigmented yeasts and saprotrophs compared to C. arabica and other Coffea species analyzed. Fungal communities associated with hybrids were more similar to those of C. arabica than to the other parental species, indicating asymmetric contributions of parental traits to the colonization of the hybrids’ phylloplane. A co-occurrence network revealed that neutral associations were more prevalent in Coffea hybrids than in Coffea species. These results indicate that while dominant fungal taxa are largely conserved across Coffea species and hybrids, interspecific hybridization is associated with the reorganization of the ecological relations in a fungal community. Overall, host genetics and hybridization-related traits influence the assembly and ecological organization of leaf-associated fungal communities in Coffea.

Microbiol Resour Announc 0:e00336-26. https://doi.org/10.1128/mra.00336-26

Here, we report draft genome sequences of four novel bacterial species from Atlantic rainforest stream sediments in southeastern Brazil. The genomes represent distinct lineages within Nitrospirota and Pseudomonadota (average nucleotide identity <95% to known species) and encode diverse metabolic capabilities, including nitrification, denitrification, and aromatic compound degradation.

DATA AVAILABILITY
Raw metagenomic reads have been deposited in the NCBI Sequence Read Archive under BioProject accession number PRJNA1429331 (SRR37485361, SRR37485358, SRR37485359, and SRR37485360). The Supplemental material has been deposited at https://doi.org/10.6084/m9.figshare.32101195.

Micael Siegert Schimmunech^a, Carolina Deuttner Neumann Barroso^a, Anderson Ferreira Da Cunha^b, Marcelo Mendes Brandão^c, Daniel Cruz^d , Karina Ishida^d, Marcelo Beltrão Molento^a,*
^a Laboratory of Veterinary Clinical Parasitology, Department of Veterinary Medicine, Federal University of Paraná, Curitiba, PR, Brazil. This email address is being protected from spambots. You need JavaScript enabled to view it. 
^b Laboratory of Biochemistry and Applied Genetics, Department of Genetics and Evolution, Federal University of São Carlos, São Carlos, Brazil.
^c Laboratory of Integrative and Systemic Biology, Center of Molecular Biology and Genetic Engineering, Universidade Estadual de Campinas, Campinas, São Paulo, Brazil.
^d World Animal Protection. São Paulo, SP, Brazil.

Braz. arch. biol. technol. 69 • 2026 • https://doi.org/10.1590/1678-4324-2026250900

Urbanization and intensive animal farming generate large volumes of waste that introduce microbial contaminants into aquatic ecosystems, increasing the risk of pathogen dissemination. We analyzed 16S rRNA-based microbial community profiles from water samples collected upstream and downstream of pig farms and urban areas in southern Brazil including Itambaracá (P1, P3), Chapecó (P2, P4), Curitiba (P5) and Joinville (P6). Proteobacteria dominated most samples, with Gammaproteobacteria enriched near pig farming sites. Urban-impacted waters showed reduced microbial diversity and clear taxonomic shifts consistent with wastewater influence. Notably, pathogenic bacteria of public health concern, including Escherichia coli O157:H7, Shigella flexneri 2a, and Pseudomonas aeruginosa, were detected in areas affected by livestock production and urban effluent discharge. These findings demonstrate that animal production systems and urban wastewater may act as interconnected sources of environmental contamination, reshaping aquatic microbiomes and introducing clinically relevant pathogens into natural waters. This convergence highlights the need for integrated monitoring and management strategies to protect ecosystem integrity and reduce human health risks.

Leandro Pio de Sousa^1,2, Ludmila dos Passos e Silva¹, Marcelo Mendes Brandão², Oliveiro Guerreiro Filho³

¹Laboratório de Interações Multitróficas e Biodiversidade, Departamento de Biologia Animal, Instituto de Biologia, Universidade Estadual de Campinas (UNICAMP),
Campinas, Brazil
²Center for Molecular Biology and Genetic Engineering (CBMEG), State University of Campinas (Unicamp), Campinas, São Paulo, 13083-875, Brazil
³Centro de Café Alcides Carvalho, Instituto Agronômico (IAC), Campinas, São Paulo, Brazil
^∗Corresponding author. E-mail: This email address is being protected from spambots. You need JavaScript enabled to view it.

Link permanente: https://url.bioinfoguy.net/fnaf133 

Coffee leaf mine, a small moth of the family Lyonetiidae, is considered one of the most important coffee pests. Despite its economic importance, almost nothing is known about its microbiome. Seeking to help fill this knowledge gap, in our study, we investigated the bacteriome of the insect (larvae and adults) and coffee leaves by sequencing the 16S rRNA gene. We found that at the phylum level  Pseudomonadota and Bacteroidota dominate. At the genus level, leaves were mainly dominated by Sphingomonas and Methylobacterium; the caterpillars were mainly dominated by Xanthomonas and Paracoccus; the adult moths were mainly dominated by Acinetobacter and Klebsiella. We estimate that the contribution to the bacteriome of adult moths comes from larvae, 50%, from leaves, 40%, and 10% from other sources. Several bacteria with the potential to degrade insecticides and secondary plant compounds were found, which leads to the expectation that they may help their hosts in resisting these toxic compounds. We hope that this work will contribute to encouraging further investigation of this important coffee pest.

Data availability

NCBI BioProject under the accession number PRJNA1004833

Karina L. Silva-Brandão¹, Julia Cabral Teresa², Clécio Fernando Klitzke, Marcelo M. Brandão³, José Roberto Trigo²

1 Leibniz Institute for the Analysis of Biodiversity Change, Museum of Nature Hamburg. Martin-Luther-King-Platz 3, 20421 Hamburg, Germany. Email: This email address is being protected from spambots. You need JavaScript enabled to view it.
2 Departamento de Biologia Animal, Instituto de Biologia, Universidade Estadual de Campinas. Rua Monteiro Lobato 255, Campinas, SP, Brazil.
3 Centro de Biologia Molecular e Engenharia Genética, Universidade Estadual de Campinas. Av. Cândido Rondon, 400, Campinas, SP, Brazil.

 Entomologia Experimentalis et Applicata 00: 1–12. https://doi.org/10.1111/eea.13589

The neotropical swallowtail butterfly Battus polydamas is a specialist on Aristolochia (Aristochiaceae). These plants are rich in natural products such as terpenoids, lignans, β-phenylethylamines (βPEA), aporphine and isoquinoline alkaloids, as well as aristolochic acids (AAs). Larvae of B. polydamas sequester some of these compounds, such as AAs, and transfer them to adults through the pupae. AAs are considered defensive compounds against natural enemies, however, the amount of AA in the larvae's diet has an effect on their performance, which may mean a cost to eating on AA-containing leaves. In the present study we evaluated the performance of B. polydamas larvae fed from 1^st instar through pupation on two host plants with different chemistry composition, A. ringens (which has several diterpenes) and A. gigantea (which has acyclic monoterpenoids and sesquiterpenoids, but no diterpenoids or AAs). Differential gene expression as response to different larval host plants was evaluated in three biological replications of gut and fat body tissues of six 5^th instar larvae. We found significant differences in the survival of larvae feeding on the two host plants; the survival in A. gigantea being significantly higher than survival in A. ringens (GLM binomial, likelihood ratio test, df = 1, χ² = 76.082, P < 0.001). In A. gigantea, 55% of the larvae persisted until pupation, while none of the larva feeding on A. ringens survived. 807 unique contigs identified by their molecular function were upregulated in the gut of larvae fed on A. ringens, while 298 were downregulated. Down-regulated contigs include genes encoding for ribosomal proteins, superoxide dismutase, P450s, UGTs, glutathione S-transferase and many proteases. Upregulated contigs comprise genes encoding for ribosomal proteins, protein farnesyltransferase, Phosphomevalonate kinase, Dolichyl-phosphate-mannose-protein mannosyltransferase 4 and O-glucosyltransferase (possibly involved in AAs metabolization). As expected, larvae of B. polydamas were strongly influenced by host plants exhibiting different concentrations of AAs, with higher concentrations leading to worse larval performance on key fitness components, such as life cycle performance attributes and larval survival. We suggest that there is a threshold of AA concentration in the host plant that larvae can tolerate, and above such a threshold the impact of plant secondary chemicals is no longer beneficial for the larvae, but negative, disrupting their detoxification mechanism.

Permanent link - https://url.bioinfoguy.net/battus2024

Data availability

It is necessary to properly cite the data repository (https://doi.org/10.25824/redu/A3GVHV) if you choose to utilise any of the data, script, or information provided in these files.