Leandro Pio de Sousa^1,2, Ludmila dos Passos e Silva¹, Marcelo Mendes Brandão², Oliveiro Guerreiro Filho³

¹Laboratório de Interações Multitróficas e Biodiversidade, Departamento de Biologia Animal, Instituto de Biologia, Universidade Estadual de Campinas (UNICAMP),
Campinas, Brazil
²Center for Molecular Biology and Genetic Engineering (CBMEG), State University of Campinas (Unicamp), Campinas, São Paulo, 13083-875, Brazil
³Centro de Café Alcides Carvalho, Instituto Agronômico (IAC), Campinas, São Paulo, Brazil
^∗Corresponding author. E-mail: Este endereço de e-mail está sendo protegido de spambots. Você precisa habilitar o JavaScript para visualizá-lo.

Link permanente: https://url.bioinfoguy.net/fnaf133 

Coffee leaf mine, a small moth of the family Lyonetiidae, is considered one of the most important coffee pests. Despite its economic importance, almost nothing is known about its microbiome. Seeking to help fill this knowledge gap, in our study, we investigated the bacteriome of the insect (larvae and adults) and coffee leaves by sequencing the 16S rRNA gene. We found that at the phylum level  Pseudomonadota and Bacteroidota dominate. At the genus level, leaves were mainly dominated by Sphingomonas and Methylobacterium; the caterpillars were mainly dominated by Xanthomonas and Paracoccus; the adult moths were mainly dominated by Acinetobacter and Klebsiella. We estimate that the contribution to the bacteriome of adult moths comes from larvae, 50%, from leaves, 40%, and 10% from other sources. Several bacteria with the potential to degrade insecticides and secondary plant compounds were found, which leads to the expectation that they may help their hosts in resisting these toxic compounds. We hope that this work will contribute to encouraging further investigation of this important coffee pest.

Data availability

NCBI BioProject under the accession number PRJNA1004833